|
| Status |
Public on Dec 31, 2011 |
| Title |
mutant RQ |
| Sample type |
RNA |
| |
|
| Source name |
embroid body with RQ mutation in BRCA1 gene
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryoid body
strain: 129S7/SvEv Brd-Hprt b-m2
|
| Treatment protocol |
Embroid bodies were collected on day 7.
|
| Growth protocol |
To generate embryoid bodies, ES cells were trypsinized and the feeder cells were removed by incubating the cells on gelatinized plate for one hour. The ES cells in supernatant were counted and diluted to 5X104 cells/ml. The cell suspension was culture in Petri dish, in DMEM-10 media. The media was changed on day 3 and later it was changed every other day.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
gene expression data from mouse embroid body with R1669Q mutation of human BRCA1
Chang_RQ_022908.CEL
|
| Data processing |
The data were analyzed with Partek Genomic Suite using default RMA method.
|
| |
|
| Submission date |
Aug 23, 2011 |
| Last update date |
Dec 31, 2011 |
| Contact name |
Suhwan Chang |
| Organization name |
National Cancer Institute
|
| Street address |
PO Box B, NCI-Frederick
|
| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE31611 |
Expression data from embryoid body with BRCA1 mutation [mRNA] |
| GSE31637 |
Tumor Suppressor BRCA1 epigenetically controls oncogenic miRNA-155 |
|
