|
| Status |
Public on Sep 01, 2011 |
| Title |
H1650-9 |
| Sample type |
RNA |
| |
|
| Source name |
NSCLC cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: non small cell lung cancer cell (NSCLC)
erlotinib sensitivity: Yes
|
| Treatment protocol |
none
|
| Growth protocol |
NSCLC cell lines were cultured for 3d in 10% serum-containing RPMI 1640 with no drug treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen Rneasy column preparation
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human U133 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Aug 24, 2011 |
| Last update date |
Sep 01, 2011 |
| Contact name |
Esther P Black |
| E-mail(s) |
penni.black@uky.edu
|
| Phone |
859-323-5898
|
| Fax |
859-257-7564
|
| Organization name |
University of Kentucky
|
| Department |
Pharmaceutical Sciences
|
| Street address |
789 S Limestone
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40536 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE31625 |
Gene Expression Patterns that Predict Sensitivity to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors in Lung Cancer Cell Lines and Human Lung Tumors |
|
