|
| Status |
Public on May 14, 2024 |
| Title |
mug174∆ cells, rep_2 |
| Sample type |
SRA |
| |
|
| Source name |
whole cell
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
tissue: whole cell
genotype: mug174 deletion
|
| Treatment protocol |
none
|
| Growth protocol |
All the S. pombe cells were grown in YES media (0.5% yeast extract, 3% glucose, and 75 mg/L of adenine, histidine, leucine, and uracil) at 32°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extracted total RNAs using MasterPure™ Yeast RNA Purification Kit (Lucigen)
NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
21R043
|
| Data processing |
The raw data was checked by FastQC v0.11.6
Clean reads were aligned to the Schizosaccharomyces japonicus genome using STAR (2.5.3a)
The expression of the transcript was calculated by FPKM using StringTie v1.3.1c
Assembly: S. japonicus genome sequencing data; https://www.japonicusdb.org/status/genome-overview
Supplementary files format and content: an excel file containing RPKM values for each gene (a total of 7001 annotated transcripts) in each sample
|
| |
|
| Submission date |
Oct 25, 2023 |
| Last update date |
May 14, 2024 |
| Contact name |
Tomoyasu Sugiyama |
| E-mail(s) |
tsugiyama@shanghaitech.edu.cn
|
| Organization name |
ShanghaiTech University
|
| Street address |
393 Middle Huaxia Road
|
| City |
Shanghai |
| ZIP/Postal code |
201210 |
| Country |
China |
| |
|
| Platform ID |
GPL30067 |
| Series (1) |
| GSE246256 |
The effect of mug174 deletion or red1 deletion mutation on gene expression in the fission yeast Schizosaccharomyces pombe |
|
| Relations |
| BioSample |
SAMN37985573 |
| SRA |
SRX22229187 |
