RNA was extracted using RNAeasy mini kit by qiagen as per the manufractures protocol
Label
NA
Label protocol
NA
Hybridization protocol
RNA (100 ng) was used for the Metabolic Pathways Panel. RNA from each sample was hybridized with the codeset for 18 hours, and 30 μL of the reaction was loaded into the nCounter cartridge and run on the nCounter SPRINT Profiler.
Scan protocol
NA
Data processing
Raw RCC files were processed using nanostring Nsolver software to generate the Raw counts. These raw counts were used to perform a differential gene expression analysis between groups using the DESeq2 algorithm in R
