|
| Status |
Public on Nov 01, 2011 |
| Title |
GIST 18 |
| Sample type |
RNA |
| |
|
| Source name |
GIST specimen 18
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: GIST
grade: high
location: stomach
age: 65
gender: M
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a mirVana miRNA isolation kit (Ambion).
|
| Label |
Cy3
|
| Label protocol |
Total RNA (100 ng) was labeled using a miRNA Labeling Reagent & Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
|
| |
|
| Hybridization protocol |
Hybridization was performed using a miRNA Labeling Reagent & Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
|
| Scan protocol |
Array was scanned with an Agilent G2565BA Microarray Scanner.
|
| Description |
miRNA expression in clinical GIST
|
| Data processing |
Agilent Feature Extraction Software (v 9.5) was used for background subtraction.
|
| |
|
| Submission date |
Aug 30, 2011 |
| Last update date |
Nov 01, 2011 |
| Contact name |
Hiromu Suzuki |
| E-mail(s) |
hsuzuki@sapmed.ac.jp
|
| Phone |
+81-11-611-2111
|
| Organization name |
Sapporo Medical University
|
| Department |
Department of Molecular Biology
|
| Street address |
S1, W17, Chuo-ku
|
| City |
Sapporo |
| State/province |
Hokkaido |
| ZIP/Postal code |
060-8556 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10406 |
| Series (1) |
| GSE31741 |
miRNA expression signatures in gastrointestinal stromal tumors (GISTs) |
|
