|
Status |
Public on Nov 01, 2011 |
Title |
GIST 24 |
Sample type |
RNA |
|
|
Source name |
GIST specimen 24
|
Organism |
Homo sapiens |
Characteristics |
tissue: GIST grade: high location: small intestine age: 37 gender: M
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using a mirVana miRNA isolation kit (Ambion).
|
Label |
Cy3
|
Label protocol |
Total RNA (100 ng) was labeled using a miRNA Labeling Reagent & Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
|
|
|
Hybridization protocol |
Hybridization was performed using a miRNA Labeling Reagent & Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
|
Scan protocol |
Array was scanned with an Agilent G2565BA Microarray Scanner.
|
Description |
miRNA expression in clinical GIST
|
Data processing |
Agilent Feature Extraction Software (v 9.5) was used for background subtraction.
|
|
|
Submission date |
Aug 30, 2011 |
Last update date |
Nov 01, 2011 |
Contact name |
Hiromu Suzuki |
E-mail(s) |
hsuzuki@sapmed.ac.jp
|
Phone |
+81-11-611-2111
|
Organization name |
Sapporo Medical University
|
Department |
Department of Molecular Biology
|
Street address |
S1, W17, Chuo-ku
|
City |
Sapporo |
State/province |
Hokkaido |
ZIP/Postal code |
060-8556 |
Country |
Japan |
|
|
Platform ID |
GPL10406 |
Series (1) |
GSE31741 |
miRNA expression signatures in gastrointestinal stromal tumors (GISTs) |
|