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| Status |
Public on Nov 05, 2023 |
| Title |
gelADSC 2 |
| Sample type |
SRA |
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| Source name |
Liver
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| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
treatment: gelADSC
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from liver tissues and cultured cells, using the RNeasy Mini Kit (Qiagen, Hilden, Germany).
Library preparation was carried out using the TruSeq Stranded mRNA Sample Prep Kit (Illumina).
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
DNBSEQ-G400 |
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| Data processing |
Sequencing was conducted on a DNBSEQ-G400 sequencer from MGI, utilizing the 100-base single-read mode. After removing adapter sequences with Trimmomatic version 0.38, the reads were aligned to the human reference genome sequences (hg19) using TopHat2 version 2.1.1.
Assembly: hg19
Supplementary files format and content: To determine expression levels, the fragments per kilobase of exon per million mapped fragments (FPKMs) were calculated by using Cufflinks version 2.2.1.
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| Submission date |
Nov 02, 2023 |
| Last update date |
Nov 05, 2023 |
| Contact name |
Hiroki Imamura |
| E-mail(s) |
himamura0214@gmail.com
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| Phone |
09096265495
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| Organization name |
Osaka University
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| Street address |
2-2 Yamada-oka
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| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
5650871 |
| Country |
Japan |
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| Platform ID |
GPL28457 |
| Series (1) |
| GSE246897 |
RNA-seq of liver tissue extracted from mouse hepatectomy models (Control group vs. gelADSC group) |
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| Relations |
| BioSample |
SAMN38082477 |
| SRA |
SRX22342214 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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