|
Status |
Public on Mar 21, 2024 |
Title |
20mo, untreated, replicate 1 |
Sample type |
SRA |
|
|
Source name |
ear
|
Organism |
Mus musculus |
Characteristics |
tissue: ear age: 20mo cell type: fibroblast strain: C57BL/6 treatment: untreated
|
Treatment protocol |
Cells were cultured in 6-well plates with 3 ml media in each well. Chemical cocktails were added to the media such that final DMSO and PBS concentration was each 0.5%. Media and treatments were replaced every 3 days.
|
Growth protocol |
Fibroblasts were cultured in DMEM/F12 media supplemented with 1X anti-anti, 10% FBS, 1X beta-mercaptoethanol, and 1X non-essential amino acids (Gibco). Cells were grown at 37°C with 5% CO2 and 3% O2.
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Cells were washed 3x with PBS, and lysed in the dish with Trizol. Isolation of total RNA was performed according to manufacturer's protocol (direct-zol, Zymo Research) Libraries were prepared with TruSeq Stranded mRNA LT Sample Prep Kit according to TruSeq Stranded mRNA Sample Preparation Guide, Part # 15031047 Rev. E mRNA-seq (polyA-enrichment)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
A1control
|
Data processing |
Fastq files were mapped to the genome using STAR v.2.7.2b Assembly: mm10 (GRCm38.p6) Supplementary files format and content: excel file containing raw counts for each sample
|
|
|
Submission date |
Nov 03, 2023 |
Last update date |
Mar 21, 2024 |
Contact name |
Wayne Mitchell |
E-mail(s) |
wmitchell2@bwh.harvard.edu
|
Organization name |
Brigham and Women's Hospital
|
Street address |
77 Avenue Louis Pasteur
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL24247 |
Series (2) |
GSE246954 |
Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenation [RNA-seq] |
GSE247199 |
Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenation |
|
Relations |
BioSample |
SAMN38086377 |
SRA |
SRX22362621 |