|
Status |
Public on Mar 07, 2024 |
Title |
Human_PBMC_1 |
Sample type |
RNA |
|
|
Source name |
MS Patient
|
Organism |
Homo sapiens |
Characteristics |
Sex: F treatment duration (hours): Untreated ms-type: SPMS-s
|
Treatment protocol |
10 SPMS patients treated with IFN-beta-1a (Rebif) for 4 hours.
|
Growth protocol |
PBMC were isolated by density gradient centrifugation (Lympholyte, Cedarlane Laboratories, Burlington, NC) within 1–4 h of phlebotomy. Cell lysates were stored at -80!C in ‘‘buffer RLT plus’’ (Qiagen) for <12 months.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was purified using RNeasy (Qiagen).
|
Label |
biotin
|
Label protocol |
Affymetrix protocol
|
|
|
Hybridization protocol |
Samples were randomized and hybridized to highly sensitive comprehensive Clariom D microarrays (ThermoFisher Scientific)
|
Scan protocol |
Samples were randomized and hybridized to highly sensitive comprehensive Clariom D microarrays (ThermoFisher Scientific)
|
Description |
RMA expression value derived from Expression Console software; core-exon analysis
|
Data processing |
Raw CEL files from arrays were imported, log2-transformed, and normalized; gene expression was analyzed as described previously (Feng et al, 2019a). Transcriptome Analysis Console (TAC) version 4.02 (ThermoFisher) identified differentially expressed genes (DEGs).
|
|
|
Submission date |
Nov 07, 2023 |
Last update date |
Mar 07, 2024 |
Contact name |
Anthony Reder |
Organization name |
University of Chicago Medicine
|
Department |
Neurology
|
Street address |
5841 S Maryland Ave
|
City |
Chicago |
State/province |
IL |
ZIP/Postal code |
60637 |
Country |
USA |
|
|
Platform ID |
GPL24539 |
Series (1) |
GSE247181 |
Novel Biomarkers and Interferon Signature in Secondary Progressive Multiple Sclerosis |
|