|
| Status |
Public on Mar 07, 2024 |
| Title |
Human_PBMC_11 |
| Sample type |
RNA |
| |
|
| Source name |
MS Patient
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: F
treatment duration (hours): Untreated
ms-type: SPMS-a
|
| Treatment protocol |
10 SPMS patients treated with IFN-beta-1a (Rebif) for 4 hours.
|
| Growth protocol |
PBMC were isolated by density gradient centrifugation (Lympholyte, Cedarlane Laboratories, Burlington, NC) within 1–4 h of phlebotomy. Cell lysates were stored at -80!C in ‘‘buffer RLT plus’’ (Qiagen) for <12 months.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using RNeasy (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol
|
| |
|
| Hybridization protocol |
Samples were randomized and hybridized to highly sensitive comprehensive Clariom D microarrays (ThermoFisher Scientific)
|
| Scan protocol |
Samples were randomized and hybridized to highly sensitive comprehensive Clariom D microarrays (ThermoFisher Scientific)
|
| Description |
RMA expression value derived from Expression Console software; core-exon analysis
|
| Data processing |
Raw CEL files from arrays were imported, log2-transformed, and normalized; gene expression was analyzed as described previously (Feng et al, 2019a). Transcriptome Analysis Console (TAC) version 4.02 (ThermoFisher) identified differentially expressed genes (DEGs).
|
| |
|
| Submission date |
Nov 07, 2023 |
| Last update date |
Mar 07, 2024 |
| Contact name |
Anthony Reder |
| Organization name |
University of Chicago Medicine
|
| Department |
Neurology
|
| Street address |
5841 S Maryland Ave
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL24539 |
| Series (1) |
| GSE247181 |
Novel Biomarkers and Interferon Signature in Secondary Progressive Multiple Sclerosis |
|
