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| Status |
Public on Mar 27, 2024 |
| Title |
Young Wild-type |
| Sample type |
SRA |
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| Source name |
Hindpaw
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| Organism |
Mus musculus |
| Characteristics |
tissue: Hindpaw
cell type: Immune cells
disease state: control
age: Young
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| Extracted molecule |
total RNA |
| Extraction protocol |
Both hindpaws were excised amputated at the ankle, and digested in a collagenase solution for 1.5-2 hours at 37°C, with agitation every 15 minutes. The resulting cell suspension and tissue remnants were filtered through a 70µm nylon filter, and the filter was rinsed to collect cells. After centrifugation and resuspension, the cells were sorted in FACS buffer (2% FBS + 0.5mM EDTA in PBS)
Library was performed according to the manufacture's instruction (single cell 3' v3 protocol, 10x Genomics)
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Description |
10x Genomics
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| Data processing |
The demultplexing, barcoded processing, gene counting and aggregation wee made using the Cell Ranger v5.0.0
Assembly: mm10
Supplementary files format and content: Tab-separated values files and matrix files
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| Submission date |
Nov 07, 2023 |
| Last update date |
Mar 27, 2024 |
| Contact name |
Rajasekaran Mahalingam |
| Organization name |
University of Texas MD Anderson Cancer Center
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| Department |
Symptom Research
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| Street address |
6565 MD Anderson Blvd
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE247211 |
Single-Cell Analysis of Age-Related Changes in Leukocytes of Diabetic Mouse Hindpaws |
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| Relations |
| BioSample |
SAMN38150263 |
| SRA |
SRX22412123 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM7885307_YWT_barcodes.tsv.gz |
30.8 Kb |
(ftp)(http) |
TSV |
| GSM7885307_YWT_features.tsv.gz |
284.1 Kb |
(ftp)(http) |
TSV |
| GSM7885307_YWT_matrix.mtx.gz |
50.2 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
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