|
| Status |
Public on Dec 22, 2023 |
| Title |
RNA-Seq-II Retinal ganglion cells, Ezh2-Y726D overexpression, replicate 2 |
| Sample type |
SRA |
| |
|
| Source name |
Retinal ganglion cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Retinal ganglion cells
genotype: C57BL/6J
|
| Treatment protocol |
AAV2-GFP or AAV2-Ezh2-Y726D was injected into the vitreous body and the optic nerve was crushed after two weeks. Three days after the optic nerve crush, retinal ganglion cells were enriched from dissociated retinal cells by fluorescence-activated cell sorting.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from retinal ganglion cells using the PicoPure RNA Isolation Kit (Thermo Fisher Scientific KIT0204) following the manufacture’s manual.
RNA-seq libraries were prepared using the TruSeq Stranded Total RNA Kit (Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
Ezh2-Y726D-2
|
| Data processing |
Raw FASTQ data were mapped to the mouse reference genome (GRCm38) using STAR aligner (version 2.7.0d) with default parameters.
The number of counts per gene was estimated using the “quantMode” command in STAR.
Assembly: GRCm38
Supplementary files format and content: tab-delimited text file including raw count for each library
|
| |
|
| Submission date |
Nov 08, 2023 |
| Last update date |
Dec 22, 2023 |
| Contact name |
Xuewei Wang |
| E-mail(s) |
xueweiwang@usf.edu
|
| Phone |
8133960977
|
| Organization name |
University of South Florida
|
| Department |
Molecular Medicine
|
| Lab |
Xuewei Wang
|
| Street address |
4001 E Fletcher Ave, ALZ 339
|
| City |
Tampa |
| State/province |
FL |
| ZIP/Postal code |
33613 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE247318 |
Histone methyltransferase Ezh2 coordinates mammalian axon regeneration via regulation of key regenerative pathways (RNA-Seq II) |
| GSE247320 |
Histone methyltransferase Ezh2 coordinates mammalian axon regeneration via regulation of key regenerative pathways |
|
| Relations |
| BioSample |
SAMN38167463 |
| SRA |
SRX22444421 |
