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Sample GSM7887111 Query DataSets for GSM7887111
Status Public on Dec 22, 2023
Title RNA-Seq-II Retinal ganglion cells, Ezh2-Y726D overexpression, replicate 2
Sample type SRA
 
Source name Retinal ganglion cells
Organism Mus musculus
Characteristics cell type: Retinal ganglion cells
genotype: C57BL/6J
Treatment protocol AAV2-GFP or AAV2-Ezh2-Y726D was injected into the vitreous body and the optic nerve was crushed after two weeks. Three days after the optic nerve crush, retinal ganglion cells were enriched from dissociated retinal cells by fluorescence-activated cell sorting.
Extracted molecule total RNA
Extraction protocol RNA was isolated from retinal ganglion cells using the PicoPure RNA Isolation Kit (Thermo Fisher Scientific KIT0204) following the manufacture’s manual.
RNA-seq libraries were prepared using the TruSeq Stranded Total RNA Kit (Illumina).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description Ezh2-Y726D-2
Data processing Raw FASTQ data were mapped to the mouse reference genome (GRCm38) using STAR aligner (version 2.7.0d) with default parameters.
The number of counts per gene was estimated using the “quantMode” command in STAR.
Assembly: GRCm38
Supplementary files format and content: tab-delimited text file including raw count for each library
 
Submission date Nov 08, 2023
Last update date Dec 22, 2023
Contact name Xuewei Wang
E-mail(s) xueweiwang@usf.edu
Phone 8133960977
Organization name University of South Florida
Department Molecular Medicine
Lab Xuewei Wang
Street address 4001 E Fletcher Ave, ALZ 339
City Tampa
State/province FL
ZIP/Postal code 33613
Country USA
 
Platform ID GPL19057
Series (2)
GSE247318 Histone methyltransferase Ezh2 coordinates mammalian axon regeneration via regulation of key regenerative pathways (RNA-Seq II)
GSE247320 Histone methyltransferase Ezh2 coordinates mammalian axon regeneration via regulation of key regenerative pathways
Relations
BioSample SAMN38167463
SRA SRX22444421

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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