|
| Status |
Public on Jun 13, 2024 |
| Title |
Larv gypsy1 insertion K27me3 C&R Repli2 |
| Sample type |
SRA |
| |
|
| Source name |
larval imaginal leg discs
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: larval imaginal leg discs
genotype: insertion gypsy
|
| Growth protocol |
Drosophila Melanogaste flies gtown at 20°C on, standard medium
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cut&Run experiments were performed as described by Kami Ahmad in protocilas.io (https://dx.doi.org/10.17504/protocols.io.umfeu3n)
Libraries were preapred according the protocol of the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
*library strategy: CUT&RUN
Libraries sequencing quality were analyzed using fastQC software.
Alignment of paired-end reads was done using bowtie2 to the dm6 reference genome.
Alignments with MAPQ smaller than 30 were discarded.
For data visualization, bigWig files were produced using bamCoverage software (deeptools), ignoring duplicate reads and applying RPKM normalization.
Assembly: dm6
Supplementary files format and content: bigwigs
|
| |
|
| Submission date |
Nov 09, 2023 |
| Last update date |
Jun 13, 2024 |
| Contact name |
Bernd Schuettengruber |
| E-mail(s) |
bernd.schuttengruber@igh.cnrs.fr
|
| Organization name |
Institute de Génétique Humaine
|
| Lab |
Cavalli
|
| Street address |
141, rue de la cardonille
|
| City |
Montpellier |
| ZIP/Postal code |
34396 |
| Country |
France |
| |
|
| Platform ID |
GPL25244 |
| Series (1) |
| GSE247377 |
A PRE loop at the dac locus acts as a topological chromatin structure that restricts and specifies enhancer promoter communication |
|
| Relations |
| BioSample |
SAMN38128667 |
| SRA |
SRX22390877 |
