|
Status |
Public on Dec 13, 2023 |
Title |
WTctrl |
Sample type |
SRA |
|
|
Source name |
dorsal skin
|
Organism |
Mus musculus |
Characteristics |
tissue: dorsal skin Sex: male condition: 8weeks, telogen genotype: WT strain: C57Bl/6 treatment: no-UVB
|
Treatment protocol |
mouse dorsal skin were irradiated with UVB for 3times, once per two days. Detailed procedures were provided in methods.
|
Extracted molecule |
total RNA |
Extraction protocol |
Fresh collected tissue was digested and immune cells were enriched by CD45+ magnetic beads. Single cell suspension were submitted to sequencing core of BRC at Cornell University for cell viability check and sequencing.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic single cell |
Library selection |
cDNA |
Instrument model |
NextSeq 2000 |
|
|
Description |
10x genomics, sample ID in multiplexed raw files is "166160_AAC55GWM5_4_WF2_11July22"
|
Data processing |
raw data were demultplexing, barcoding and gene count using Cellranger software Assembly: mm10 Supplementary files format and content: matrix files, barcoding files and features files were provided following standard cellranger procedure.
|
|
|
Submission date |
Nov 14, 2023 |
Last update date |
Dec 13, 2023 |
Contact name |
Luye An |
E-mail(s) |
la389@cornell.edu
|
Organization name |
cornell university
|
Department |
biomedical sciences
|
Lab |
Andrew white lab
|
Street address |
618 tower road
|
City |
ithaca |
ZIP/Postal code |
14850 |
Country |
USA |
|
|
Platform ID |
GPL30172 |
Series (2) |
GSE247694 |
Sexual dimorphism in melanocyte stem cell behavior reveals combinational therapeutic strategies for cutaneous repigmentation [scRNA-seq] |
GSE247697 |
Sexual dimorphism in melanocyte stem cell behavior reveals combinational therapeutic strategies for cutaneous repigmentation |
|
Relations |
BioSample |
SAMN38248664 |
SRA |
SRX22520159 |