|
| Status |
Public on Sep 02, 2011 |
| Title |
H413 |
| Sample type |
RNA |
| |
|
| Source name |
oral squamous cell carcinoma-derived cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H413
disease state: Oral cancer
|
| Biomaterial provider |
Department of Oral and Dental Science, University of Bristol
|
| Treatment protocol |
Tumour-derived cell line was established by single cell suspension from fresh surgical biopies of untreated human oral squamous cell carcinoma.
|
| Growth protocol |
Tumour-derived cell line was cultured in DMEM/F12 (1:1) containing 10% fetal bovine serum, L-glutamine and hydrocortisone.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using the RNeasy Total
RNA Mini Kit (Qiagen, Crawley, West Sussex, UK).
|
| Label |
biotin
|
| Label protocol |
The cDNA was synthesized, in vitro transcribed and labeled using standard Affymetrix protocols.
|
| |
|
| Hybridization protocol |
Affymetrix Standard protocol
|
| Scan protocol |
Affymetrix Standard protocol
|
| Data processing |
The scanned images of microarray chips were analyzed using the Affymetrix GenChip Operating Software (GCOS) and Expression Console with the default settings except that the target signal was set to 100.
|
| |
|
| Submission date |
Sep 02, 2011 |
| Last update date |
Sep 02, 2011 |
| Contact name |
Wenbin Wei |
| E-mail(s) |
Wenbin.Wei2@durham.ac.uk
|
| Organization name |
Durham University
|
| Department |
Biosciences
|
| Street address |
Stockton Road
|
| City |
Durham |
| ZIP/Postal code |
DH1 3LE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE31853 |
Expression data from oral squamous cell carcinoma (OSCC)-derived cell lines and normal oral keratinocytes |
|
