|
Status |
Public on May 01, 2024 |
Title |
RNA-Seq of mus musculus: adult female brain,20μm |
Sample type |
SRA |
|
|
Source name |
Brain
|
Organism |
Mus musculus |
Characteristics |
tissue: Brain Sex: Female age: 12 weeks genotype: C57BL/6J treatment: Cryosection
|
Extracted molecule |
total RNA |
Extraction protocol |
Tissue was permeabilized with tissue permeabilizing enzyme for 12 minutes RNA libraries for RNA-seq were prepared using Kapa HIFI HotStart ReadyMix PCR Kit following manufacturer's protocols.
|
|
|
Library strategy |
OTHER |
Library source |
transcriptomic |
Library selection |
other |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
Well-ST-seq
|
Data processing |
*library strategy: Well-ST-seq The filtered data was mapped to the mouse genome (mm10 mouse) using SpaceRanger V1.1.0. Assembly: mm10 Supplementary files format and content: Tab-separated values files and matrix files
|
|
|
Submission date |
Nov 15, 2023 |
Last update date |
Jun 06, 2024 |
Contact name |
Zhengyang Jin |
E-mail(s) |
jinzy21@mails.jlu.edu.cn
|
Organization name |
Jilin University
|
Street address |
No. 1163 Xinmin Street
|
City |
Changchun |
State/province |
Jilin |
ZIP/Postal code |
130021 |
Country |
China |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE247834 |
Well-ST-seq: cost-effective and cellular level spatial transcriptomics with high RNA capture efficiency |
|
Relations |
SRA |
SRX22516731 |
BioSample |
SAMN38241837 |