|
| Status |
Public on Mar 22, 2013 |
| Title |
TGFb1-Tg lung Replicate 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Control B6J WT Replicates combined
|
| Organism |
Mus musculus |
| Characteristics |
treatment: control
age: 10 weeks
genotype: wild type
tissue: Lung
genetic background: B6J
|
| Growth protocol |
8-10 week old transgenic (Tg) mice and wild-type (WT) littermate controls were used in all experiments. To induce expression of active hTGF-β1 in the lungs of transgenic mice, doxycycline (DOX) was added to the drinking water at 1 mg/ml. Lungs were harvested at various time points, ranging from 2 to 14 days, following the addition of DOX to the drinking water (i.e. hTGF-β1 induction).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
100 ng-input two-round RT/IVT RNA amplification protocol
|
| |
|
| Channel 2 |
| Source name |
TGFb1-Tg lung Replicate 1
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: B6J
genotype: TGFb1-Tg
treatment: TGFb1-Tg overexpression
age: 10 weeks
tissue: Lung
|
| Growth protocol |
8-10 week old transgenic (Tg) mice and wild-type (WT) littermate controls were used in all experiments. To induce expression of active hTGF-β1 in the lungs of transgenic mice, doxycycline (DOX) was added to the drinking water at 1 mg/ml. Lungs were harvested at various time points, ranging from 2 to 14 days, following the addition of DOX to the drinking water (i.e. hTGF-β1 induction).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
100 ng-input two-round RT/IVT RNA amplification protocol
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
|
| Description |
TGFb1-Tg lung Replicate 1
|
| Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Sep 07, 2011 |
| Last update date |
Mar 22, 2013 |
| Contact name |
I-Ming Wang |
| E-mail(s) |
i_ming_wang@merck.com
|
| Phone |
215-652-1287
|
| Organization name |
Merck Research Lab
|
| Department |
Genetics and Pharmacogenomics
|
| Lab |
Discovery Pharmacogenomics
|
| Street address |
770 Sumneytown Pike
|
| City |
West Point |
| State/province |
PA |
| ZIP/Postal code |
19486 |
| Country |
USA |
| |
|
| Platform ID |
GPL3677 |
| Series (2) |
| GSE31559 |
Various mice and rat tissues subjected to various treatments |
| GSE31950 |
Profiling fibrotic lung in TGFb1-Tg mice |
|
