|
| Status |
Public on Jun 18, 2012 |
| Title |
H650 |
| Sample type |
RNA |
| |
|
| Source name |
Lung cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: lung cancer cell line
cell line: H650
atcc alias: CRL-5835
histology: Adenocarcinoma
age: --
race: Z
gender: M
|
| Biomaterial provider |
ATCC: CRL-5835
|
| Treatment protocol |
None
|
| Growth protocol |
RPMI 5% BFS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit including DNase1 treatment (lung cancer cell lines and HBECs); Trizol (HSAECs)
|
| Label |
biotin
|
| Label protocol |
500 ng of total RNA was used to label the cRNA probes by Ambion Illumina TotalPrep RNA Amplification kit (Cat# IL1791)
|
| |
|
| Hybridization protocol |
1.5 ug of the amplified and labeled cRNA probes was hybridized to the chip overnight at 58 degree C, then washed, blocked and detected by streptavidin-Cy3 per manufacturer's protocol.
|
| Scan protocol |
The chips were scanned by Illumina iScan system.
|
| Data processing |
Bead-level data were processed with the MBCB algorithm (Ding et al, Nucl Acids Res, 36:e58, 2008), which is a background-correction and summarization method, then quantile-normalized and log2-transformed
|
| |
|
| Submission date |
Sep 09, 2011 |
| Last update date |
Jun 18, 2012 |
| Contact name |
Luc Girard |
| E-mail(s) |
Luc.Girard@UTSouthwestern.edu
|
| Organization name |
UT Southwestern Medical Center
|
| Department |
Hamon Center for Therapeutic Oncology Research
|
| Lab |
NB8.114A
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-8593 |
| Country |
USA |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE32036 |
Expression profiling of lung cancer cell lines |
|
