|
Status |
Public on Jun 18, 2012 |
Title |
H889 |
Sample type |
RNA |
|
|
Source name |
Lung cancer cell line
|
Organism |
Homo sapiens |
Characteristics |
cell type: lung cancer cell line cell line: H889 atcc alias: CRL-5817 histology: SCLC age: 69 race: Caucasian gender: F
|
Biomaterial provider |
ATCC: CRL-5817
|
Treatment protocol |
None
|
Growth protocol |
RPMI 5% BFS
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy kit including DNase1 treatment (lung cancer cell lines and HBECs); Trizol (HSAECs)
|
Label |
biotin
|
Label protocol |
500 ng of total RNA was used to label the cRNA probes by Ambion Illumina TotalPrep RNA Amplification kit (Cat# IL1791)
|
|
|
Hybridization protocol |
1.5 ug of the amplified and labeled cRNA probes was hybridized to the chip overnight at 58 degree C, then washed, blocked and detected by streptavidin-Cy3 per manufacturer's protocol.
|
Scan protocol |
The chips were scanned by Illumina iScan system.
|
Data processing |
Bead-level data were processed with the MBCB algorithm (Ding et al, Nucl Acids Res, 36:e58, 2008), which is a background-correction and summarization method, then quantile-normalized and log2-transformed
|
|
|
Submission date |
Sep 09, 2011 |
Last update date |
Jun 18, 2012 |
Contact name |
Luc Girard |
E-mail(s) |
Luc.Girard@UTSouthwestern.edu
|
Organization name |
UT Southwestern Medical Center
|
Department |
Hamon Center for Therapeutic Oncology Research
|
Lab |
NB8.114A
|
Street address |
5323 Harry Hines Blvd
|
City |
Dallas |
State/province |
TX |
ZIP/Postal code |
75390-8593 |
Country |
USA |
|
|
Platform ID |
GPL6884 |
Series (1) |
GSE32036 |
Expression profiling of lung cancer cell lines |
|