|
| Status |
Public on Nov 23, 2011 |
| Title |
Serum samples of patients with Parkinson’s disease (PD) 8-1 |
| Sample type |
protein |
| |
|
| Source name |
serum
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Parkinson's disease PD
block in gpr file: 5
sample type: serum
|
| Extracted molecule |
protein |
| Extraction protocol |
serum samples
|
| Label |
Cy3
|
| Label protocol |
According to manufacturer's protocols. In brief, 70ul of 1,500 fold diluted Fluorescent dye-conjugated streptavidin ws added to each subarray and incubated in a dark room at room temperature for 1-2 hours.
|
| |
|
| Hybridization protocol |
The array consisted of three glass slides (array 6, 7, and 8) that were pretreated according to the manufacturer's instructions and incubated with 2-fold diluted serum pools for 2 hours. All sample measurements were performed in duplicate. The array glass slides were washed, incubated with a biotin-conjugated anti-cytokine mix for 2 hours, washed again, and developed for 2 hours with Cy3-conjugated streptavidin.
|
| Scan protocol |
Fluorescence intensities (Cy3) were recorded with an Axon GenePix 4000B scanner.
|
| Description |
pool A of 10 serum samples
|
| Data processing |
Raw data was analyzed with the Raybiotech analysis tool, a data analysis program based on Microsoft Excel technology specifically designed for the analysis of these microarrays. Signals were normalized using internal, positive and negative controls included on the array. Signals were normalized using positive and negative control features on the microarray, the fold changes are relative expression to positive control features, i.e. a value of 1 is same expression than positive control, 2 a 2 fold higher, 0.5 a 2-fold lower expression.
|
| |
|
| Submission date |
Sep 09, 2011 |
| Last update date |
Nov 24, 2011 |
| Contact name |
Johannes Rainer |
| E-mail(s) |
johannes.rainer@eurac.edu
|
| Organization name |
Eurac Researc
|
| Department |
Institute for Biomedicine
|
| Lab |
Biomedical Informatics
|
| Street address |
Via A. Volta 21
|
| City |
Bolzano |
| ZIP/Postal code |
39100 |
| Country |
Italy |
| |
|
| Platform ID |
GPL14561 |
| Series (2) |
| GSE32040 |
Identification of potential biomarkers for patients with neurodegenerative parkinsonian syndromes using serum cytokine microarray analysis; series 8 |
| GSE32041 |
Identification of potential biomarkers for patients with neurodegenerative parkinsonian syndromes using serum cytokine microarray analysis |
|
