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Status
Public on Mar 14, 2012
Title
Spl4-10_rep1
Sample type
RNA
Source name
CD4 T cell clone
Organism
Mus musculus Characteristics
cd4 t cell clone: Spl4-10
Treatment protocol
harvested at 72 hours
Growth protocol
Usual growth medium (RPMI 1640; 10^-5M 2 mercaptoethanol; 1x glutamax-1 (gibco); recombinant IL-1a, IL-2, IL-6, IL-7, IL-15, TNF-a, IFN-4a, IFN-b)
Extracted molecule
total RNA
Extraction protocol
RNAeasy with DNAse1 treatment step (Qiagen)
Label
biotin
Label protocol
RNA was labeled using Affymetrix standard WT protocol (non-Ambion) using 100ng total RNA.
Hybridization protocol
Hybridization was done using Affymetrix standard hybridization protocol for Gene ST 1.0 arrays
Scan protocol
Scanned using GCOS
Data processing
Partek was used to generate RMA.RMA signals were generated for the core probe sets using the RMA background correction, Quantile normalization and summarization by Median Polish. Summarized signals for each probe set were log2 transformed.
Submission date
Sep 14, 2011
Last update date
Mar 14, 2012
Contact name
Raymond Morris Johnson
E-mail(s)
raymond.johnson@yale.edu Organization name
Yale University
Department
Med/Infectious Diseases
Street address
PO Box 208022
City
New Haven
State/province
CT
ZIP/Postal code
06520-8022
Country
USA
Platform ID
GPL6246 Series (1)
GSE32128 Plac8-dependent and iNOS-dependent T cell-mediate mechanisms clear Chlamydia muridarum infections from the genital tract
Data table header descriptions ID_REF VALUE RMA log base 2 transformed
Data table ID_REF VALUE 10344614
6.04184
10344616
2.58994
10344618
2.99768
10344620
3.67041
10344622
6.7492
10344624
10.6199
10344633
9.87441
10344637
8.40349
10344653
3.76667
10344658
8.38331
10344674
3.39189
10344679
5.07038
10344705
5.1491
10344707
9.48413
10344713
10.3427
10344715
3.37576
10344717
4.5037
10344719
5.67998
10344721
2.48738
10344723
7.51623
28853 475 Kbytes .Supplementary file Size Download File type/resource GSM796421.CEL.gz
4.2 Mb
(ftp) (http) CEL
Processed data included within Sample table
