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Status
Public on Mar 14, 2012
Title
uvmo-2_rep1
Sample type
RNA
Source name
CD4 T cell clone
Organism
Mus musculus Characteristics
cd4 t cell clone: uvmo-2
Treatment protocol
harvested at 72 hours
Growth protocol
Usual growth medium (RPMI 1640; 10^-5M 2 mercaptoethanol; 1x glutamax-1 (gibco); recombinant IL-1a, IL-2, IL-6, IL-7, IL-15, TNF-a, IFN-4a, IFN-b)
Extracted molecule
total RNA
Extraction protocol
RNAeasy with DNAse1 treatment step (Qiagen)
Label
biotin
Label protocol
RNA was labeled using Affymetrix standard WT protocol (non-Ambion) using 100ng total RNA.
Hybridization protocol
Hybridization was done using Affymetrix standard hybridization protocol for Gene ST 1.0 arrays
Scan protocol
Scanned using GCOS
Data processing
Partek was used to generate RMA.RMA signals were generated for the core probe sets using the RMA background correction, Quantile normalization and summarization by Median Polish. Summarized signals for each probe set were log2 transformed.
Submission date
Sep 14, 2011
Last update date
Mar 14, 2012
Contact name
Raymond Morris Johnson
E-mail(s)
raymond.johnson@yale.edu Organization name
Yale University
Department
Med/Infectious Diseases
Street address
PO Box 208022
City
New Haven
State/province
CT
ZIP/Postal code
06520-8022
Country
USA
Platform ID
GPL6246 Series (1)
GSE32128 Plac8-dependent and iNOS-dependent T cell-mediate mechanisms clear Chlamydia muridarum infections from the genital tract
Data table header descriptions ID_REF VALUE RMA log base 2 transformed
Data table ID_REF VALUE 10344614
6.06152
10344616
2.60596
10344618
2.86961
10344620
3.48263
10344622
6.79982
10344624
10.2623
10344633
10.1103
10344637
7.67256
10344653
3.79166
10344658
8.9716
10344674
3.15422
10344679
4.80646
10344705
5.3174
10344707
9.63764
10344713
10.2928
10344715
3.38961
10344717
4.30269
10344719
5.85278
10344721
2.51074
10344723
7.78389
28853 475 Kbytes .Supplementary file Size Download File type/resource GSM796425.CEL.gz
4.2 Mb
(ftp) (http) CEL
Processed data included within Sample table
