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Status
Public on Mar 14, 2012
Title
uvmo-2_rep2
Sample type
RNA
Source name
CD4 T cell clone
Organism
Mus musculus Characteristics
cd4 t cell clone: uvmo-2
Treatment protocol
harvested at 72 hours
Growth protocol
Usual growth medium (RPMI 1640; 10^-5M 2 mercaptoethanol; 1x glutamax-1 (gibco); recombinant IL-1a, IL-2, IL-6, IL-7, IL-15, TNF-a, IFN-4a, IFN-b)
Extracted molecule
total RNA
Extraction protocol
RNAeasy with DNAse1 treatment step (Qiagen)
Label
biotin
Label protocol
RNA was labeled using Affymetrix standard WT protocol (non-Ambion) using 100ng total RNA.
Hybridization protocol
Hybridization was done using Affymetrix standard hybridization protocol for Gene ST 1.0 arrays
Scan protocol
Scanned using GCOS
Data processing
Partek was used to generate RMA.RMA signals were generated for the core probe sets using the RMA background correction, Quantile normalization and summarization by Median Polish. Summarized signals for each probe set were log2 transformed.
Submission date
Sep 14, 2011
Last update date
Mar 14, 2012
Contact name
Raymond Morris Johnson
E-mail(s)
raymond.johnson@yale.edu Organization name
Yale University
Department
Med/Infectious Diseases
Street address
PO Box 208022
City
New Haven
State/province
CT
ZIP/Postal code
06520-8022
Country
USA
Platform ID
GPL6246 Series (1)
GSE32128 Plac8-dependent and iNOS-dependent T cell-mediate mechanisms clear Chlamydia muridarum infections from the genital tract
Data table header descriptions ID_REF VALUE RMA log base 2 transformed
Data table ID_REF VALUE 10344614
6.35384
10344616
2.59252
10344618
2.81257
10344620
3.78042
10344622
6.74234
10344624
10.4815
10344633
10.1884
10344637
7.54597
10344653
3.80243
10344658
8.6524
10344674
3.10135
10344679
4.85894
10344705
4.84739
10344707
9.39549
10344713
10.5529
10344715
3.7818
10344717
4.71825
10344719
5.45247
10344721
2.55989
10344723
7.51608
28853 475 Kbytes .Supplementary file Size Download File type/resource GSM796426.CEL.gz
4.1 Mb
(ftp) (http) CEL
Processed data included within Sample table
