|
| Status |
Public on Jan 04, 2012 |
| Title |
DOX, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
ErbB3 DOX-KO
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB
genotype/variation: ErbB3 DOX-KO
tissue: Mammary epithelial organoids
treatment: DOX
|
| Treatment protocol |
Mammary epithelial organoids harvested from age-matched female mice were plated in growth factor-reduced Matrigel ± 5 µM DOX, cultured for 14 d
|
| Growth protocol |
The mammary glands of ErbB3 DOX-KO mice were harvested under sterile conditions, minced, and digested in 1 mg/ml collagenase/0.3 mg/ml hyaluronidase (Stem Cell Technologies, Vancouver, Canada). Organoids were processed according to protocol provided by Stem Cell Technologies, resuspended in growth factor-reduced Matrigel (BD Life Sciences) and plated in 8-well chamber slides.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Affy-FS450-0007
|
| |
|
| Hybridization protocol |
Affy-169 : 300.0 ng at 45 degree_C during 17 hours
|
| Scan protocol |
Arrays were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
gene expression data from organoids treated with DOX
|
| Data processing |
Data were processed using RMA normalization in R using the affy package and the justRMA function
|
| |
|
| Submission date |
Sep 14, 2011 |
| Last update date |
Jan 04, 2012 |
| Contact name |
Justin M Balko |
| Organization name |
Vanderbilt University
|
| Department |
Medicine
|
| Lab |
Arteaga
|
| Street address |
777 Preston Research Building
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232-6307 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE32129 |
Targeted ErbB3 loss in mammary organoids harvested from ErbB3 DOX-KO mice +/- DOX treatment |
|
