|
| Status |
Public on Dec 19, 2023 |
| Title |
PRP_difer6w_2 |
| Sample type |
RNA |
| |
|
| Source name |
pBM-MSC gelatin-PRP microspheres in chondrogenic differentiation medium for 6 weeks of culture, biological rep 2
|
| Organism |
Sus scrofa |
| Characteristics |
cell type: Sus scrofa Bone Marrow Mesenchymal Stem Cells
material: gelatin-PRP
culture media: chondrogenic differentiation
culture time: 6 weeks
|
| Treatment protocol |
Cells were seeded in gelatin or gelatin-PRP microspheres and cultured with proliferation and chondrogenic differentiation media (Gibco).
|
| Growth protocol |
Cultures were maintained for 6 weeks at 37 ºC and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The miRNA extracts were labeled with biotin, using 3DNA Array Detection FlashTag™ Biotin HSR (Genisphere, Hatfield, USA) following standardized protocols provided by Affymetrix.
|
| |
|
| Hybridization protocol |
The biotinylated RNA were hybridized with the microarrays in a 645-hybridization oven (Affymetrix, CA, USA), following standardized protocols provided by Affymetrix.
|
| Scan protocol |
The samples were performed using a Fluidic 450 Station and a GeneChip 3000 7G confocal scanner (Affymetrix, CA, USA).
|
| Description |
microRNA expression data from pBM-MSC gelatin-PRP microspheres in chondrogenic differentiation medium for 6 weeks of culture
|
| Data processing |
MicroRNA expressions were analyzed using Transcriptome Analysis Console (TAC) Software (Applied Biosystems).
|
| |
|
| Submission date |
Dec 14, 2023 |
| Last update date |
Dec 19, 2023 |
| Contact name |
Rubén Salvador-Clavell |
| E-mail(s) |
ruben.salvador@uv.es
|
| Organization name |
University of Valencia
|
| Street address |
13 Blasco Ibanez Av.
|
| City |
Valencia |
| State/province |
Valencia |
| ZIP/Postal code |
46010 |
| Country |
Spain |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE250185 |
miRNA expression data from Sus scrofa BM-MSC |
|
