|
| Status |
Public on Dec 18, 2023 |
| Title |
TB-KSF06 |
| Sample type |
RNA |
| |
|
| Source name |
advanced NSCLC
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: NSCLC tumor
age: 77
gender: Male
smoking: current or former
ecog ps: 1
pd-l1 tps: 80
histology: Adeno
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tumor tissue was microdissected to avoid contamination from nontumor tissue before extraction of intratumoral RNA with the use of an AllPrep DNA/RNA FFPE Kit (Qiagen). The extracted RNA was measured with a NanoDrop system (Thermo Fisher Scientific) and an Agilent 2100 Bioanalyzer system (Agilent Technologies).
|
| Label |
Nanostring barcoding
|
| Label protocol |
RNA were directly tagged with a capture probe and a reporter probe. This process was carried out according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Hybridation was performed as per manufacturer's protocol.
|
| Scan protocol |
Scanned with nCounter SPRINT Profiler
|
| Description |
Pancancer IO 360 panel
|
| Data processing |
Data was normalised using the nSolver software, version 4.0.
|
| |
|
| Submission date |
Dec 15, 2023 |
| Last update date |
Dec 18, 2023 |
| Contact name |
Takashi Kurosaki |
| E-mail(s) |
1955c7@med.kindai.ac.jp
|
| Organization name |
kindai university
|
| Street address |
377-2 Ohno-higashi
|
| City |
Osakasayama City |
| ZIP/Postal code |
589-8511 |
| Country |
Japan |
| |
|
| Platform ID |
GPL27956 |
| Series (1) |
| GSE250262 |
Association of tumor burden with the efficacy of PD-(L)1 inhibitors for treatment-naive advanced non-small-cell lung cancer |
|
