|
| Status |
Public on Feb 09, 2024 |
| Title |
6wk_kaku4-2 |
| Sample type |
SRA |
| |
|
| Source name |
6-week-old leaves
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: 6-week-old leaves
genotype: kaku4-2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIZOL® reagent (Invitrogen, CA, USA) and purified using Qiagen RNeasy columns (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.
Sequencing libraries were constructed by the Beijing Genomics Institute and sequenced using an IlluminaHiSeq™ 2000 with 150-bp pair-end reads.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
RNA-seq_FPKM_matrix.txt
|
| Data processing |
RNA-seq data with 3 biological replicates were generated. The paired-end clean reads of RNA-seq were aligned to the reference genome (TAIR10) by TopHat2 v2.0.9 software. FPKM values were calculated using Cufflinks.
Assembly: Arabidopsis reference genome (TAIR10)
Supplementary files format and content: RNA-seq_FPKM_matrix
|
| |
|
| Submission date |
Dec 15, 2023 |
| Last update date |
Feb 09, 2024 |
| Contact name |
Zhen Su |
| E-mail(s) |
zhensu@cau.edu.cn
|
| Phone |
+86-10-62734385
|
| Organization name |
China Agricultural University
|
| Department |
College of Biological Sciences
|
| Street address |
#2 Yuanmingyuan Xilu
|
| City |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platform ID |
GPL13222 |
| Series (1) |
| GSE250299 |
Transcriptome and epigenome data analysis to reveal the key role of KAKU4 during leaf senescence in Arabidopsis |
|
| Relations |
| BioSample |
SAMN38859576 |
| SRA |
SRX22906797 |
