|
| Status |
Public on Dec 07, 2011 |
| Title |
4T1-TB_1 |
| Sample type |
RNA |
| |
|
| Source name |
Splenic CD11b+Gr-1high cell populations
|
| Organism |
Mus musculus |
| Characteristics |
strain: BALB/c
gender: female
experimental group: 4T1-TB (tumor-bearing)
tissue: lymphoid
cell type: Splenic CD11b+Gr-1high cell populations
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from highly purified splenic CD11b+Gr-1high cells from the different experimental groups was quantified by a ND-1000 spectrophotometer (NanoDrop, Thermo Scientific) and evaluated for degradation using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Samples requirements were as follows: RIN >7, an OD 260:280 of 1.9-2.0, and an OD 260/230 >1.8.
|
| Label |
biotin
|
| Label protocol |
Initially, we converted 250-500 ng total RNA to cDNA, followed by in vitro transcription to generate biotin-labeled cRNA (Illumina TotalPrep RNA Amplification Kit, Ambion Inc., Austin, TX).
|
| |
|
| Hybridization protocol |
The labeled probes were then mixed with hybridization reagents and hybridized overnight to the MouseWG-6 v2 BeadChips.
|
| Scan protocol |
Following washing and staining, the BeadChips were imaged using the Illumina BeadArray Reader to measure fluorescence intensity at each probe.
|
| Data processing |
BeadChip data files are analyzed with Illumina’s GenomeStudio gene expression module (V1.6.0)
|
| |
|
| Submission date |
Sep 19, 2011 |
| Last update date |
Dec 07, 2011 |
| Contact name |
song liu |
| Organization name |
Roswell Park Cancer Institute
|
| Street address |
Elm and Carlton Streets
|
| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14263 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE32209 |
Tumor-Derived G-CSF Facilitates Neoplastic Growth through a Granulocytic Myeloid-Derived Suppressor Cell-Dependent Mechanism |
|
