|
| Status |
Public on Jan 01, 2012 |
| Title |
Cochlea, biol rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Chick inner ear
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: Cochlea
age: Embryonic day 20-21
|
| Treatment protocol |
Sensory epithelia from cohclea and utricle were microdissected, leaving pure populations of hair cells and supporting cells.
|
| Growth protocol |
Fertilized chicken eggs were incubated at 38°C for 20 to 21 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from 10-20 pooled utricle or cochlear epithelia using Trizol and RNeasy MinElute Cleanup Kit (Qiagen) using the manufacturers' instructions.
|
| Label |
biotin
|
| Label protocol |
The sample was amplified and labeled using the Ambion MessageAmp Premier RNA Amplification/Affx fragmentation (Ambion M.Amp_Affy frag) protocol. Note that a nonstandard labeling input amount of 0.08μg was used.
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
The microarray was incubated, washed, and read by a Microcon Array imager
|
| Description |
Gene expression data from auditory organ at E20-21
|
| Data processing |
GC-RMA, quantile normalization, and summarized using a linear model estimated by the median polish algorithm.
|
| |
|
| Submission date |
Sep 21, 2011 |
| Last update date |
Jan 01, 2012 |
| Contact name |
Peter Gillespie |
| E-mail(s) |
gillespp@ohsu.edu
|
| Phone |
503 494 2936
|
| Fax |
503 494 2976
|
| Organization name |
Oregon Health & Science University
|
| Department |
Oregon Hearing Research Center
|
| Lab |
Gillespie
|
| Street address |
L335A / 3181 SW Sam Jackson Pk Rd
|
| City |
Portland |
| State/province |
OR |
| ZIP/Postal code |
97239 |
| Country |
USA |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE32272 |
Expression data from chick cochlea and utricle |
|
