Adult male and female C57BL/6J mice were exposed to electronic cigarettes with 0%, 3% and 6% nicotine daily (70 ml, 3.3s, 1 puff per min/30 min) for 14 days, using the inExpose full body chamber (SCIREQ). Following exposure, lung tissue was harvested and RNA extracted.
Growth protocol
n/a
Extracted molecule
total RNA
Extraction protocol
Lung tissue was homogenized in Trizol. Total RNA was extracted with the Direct-zol (Zymo Research)
Label
SYBR Green
Label protocol
A total of 100 ng of purified RNA were retrotranscribed with the RT2 First Strand Kit (Qiagen). The expression of 84 NRs genes and coregulators was measured with the RT² ProfilerTM Array Mouse Receptors & Coregulators (PAMM-056YE)
Hybridization protocol
n/a
Scan protocol
n/a
Description
male mouse not exposed (control)
Data processing
Data were analyzed in excel using Ct values for each sample, normalized to the average Ct of 5 endogenous controls (Actb, B2m, Gapdh, Gusb, Hsp90ab1)
