|
| Status |
Public on Jan 03, 2024 |
| Title |
SAMPLE 8 Day1postIR NHP |
| Sample type |
protein |
| |
|
| Source name |
Plasma
|
| Organism |
Macaca mulatta |
| Characteristics |
dose: ~3.5GY
time point: Day1postIR
|
| Treatment protocol |
2 male and 1 female Rhesus monkeys (Macaca mulatta) between 5–8 years of age received a single dose of TBI between 3.85 and 4.22 Gy.
|
| Extracted molecule |
protein |
| Extraction protocol |
Plasma samples were collected at 24 h postirradiation using venipuncture collection.
|
| Label |
cy3
|
| Label protocol |
SomaScan assay protocol
|
| |
|
| Hybridization protocol |
SomaLogic
|
| Scan protocol |
SomaLogic
|
| Description |
SAMPLE 8
|
| Data processing |
data were received in the form of Relative Fluorescent Units (RFU) for each of the 1,310 proteins in the SomaScan assay after normalizing for intraplate and interplate variation using the supplied SomaLogic software. These RFU scores for each protein were log2 and z-score transformed. Mean control expression values were compared to each individual radiation treated sample for the murine and clinical samples. Matched sample pre- and postirradiation were used for the NHP and minipig animals. The ratios of respective upregulation or downregulation of each protein was filtered using a (p < 0.05) and 1.3-fold change threshold.
|
| |
|
| Submission date |
Jan 03, 2024 |
| Last update date |
Jan 03, 2024 |
| Contact name |
Uma T Shankavaram |
| E-mail(s) |
uma@mail.nih.gov
|
| Phone |
301-496-6718
|
| Organization name |
NIH
|
| Department |
NCI
|
| Lab |
Radiation Oncology Branch
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL33741 |
| Series (1) |
| GSE252430 |
Comparison of Proteomic Expression Profiles after Radiation Exposure across Four Different Species |
|
