|
| Status |
Public on May 01, 2024 |
| Title |
KO1 IP |
| Sample type |
SRA |
| |
|
| Source name |
Cortex/Hippocampus
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Cortex/Hippocampus
genotype: Mettl14 KO
treatment: m6A IP
|
| Growth protocol |
DMEM/F12 + B27/N2/Glutamine. Cultured at 37C and 5% CO2
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was extracted using Trizol and submitted to library building. m6A-IP samples were subjected to immunoprecipitation using an anti-m6A antibody prior to library building.
Libraries were built using the Illumina TruSeq Stranded mRNA Library Prep Kit.
|
| |
|
| Library strategy |
RIP-Seq |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
KO_con_peak.bed
|
| Data processing |
STAR was used for read alignments. exomePeak was used for peak calling. MemeChip was used for motif analysis. Ontology was performed with DAVID. Meta-analysis was performed using MetaPlot.
Assembly: GRCm38
Supplementary files format and content: Consistent Peaks across replicates are presented as .bed files for the WT and KO. Genes for each peak are listed with peak coordinates as well.
|
| |
|
| Submission date |
Jan 03, 2024 |
| Last update date |
May 01, 2024 |
| Contact name |
Michael Kahl |
| E-mail(s) |
mkahl@u.northwestern.edu
|
| Organization name |
Northwestern University
|
| Street address |
303 E Superior St
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL21103 |
| Series (1) |
| GSE252477 |
m6A RNA Methylation Regulates Mitochondrial Function |
|
| Relations |
| BioSample |
SAMN39244500 |
| SRA |
SRX23085831 |
