|
| Status |
Public on Feb 04, 2024 |
| Title |
OE-TabHLH489_5DPA_grain_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Grain
|
| Organism |
Triticum aestivum |
| Characteristics |
tissue: Grain
genotype: TabHLH489 overexpression
time: 5 days post anthesis
|
| Growth protocol |
The plants were cultivated in a greenhouse under a 16-hour light (22 °C)/8-hour dark (16 °C) cycle, maintaining approximately 50% relative humidity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from grains using M5 HiPer Plant RNeasy Complex Mini Kit (Mei5bio). 1 μg total RNA was used for the construction of sequencing libraries.
The sequencing libraries construction and sequencing on the BGISEQ-500 platform were performed at Beijing Geonomics institute(BGI)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Data processing |
The RNA-seq clean reads were mapped to the reference genome IWGSC Refseq v1.1 using Bowtie2
Htseq-count with default parameters was used to calculate the gene read counts
The gene read counts were normalized to TPM and calculated the gene expression level by Salmon.
Assembly: IWGSC_Refseq_v1.1
Supplementary files format and content: text file includes read counts of genes for each sample
Supplementary files format and content: text file includes TPM value of genes for each sample
|
| |
|
| Submission date |
Jan 16, 2024 |
| Last update date |
Feb 04, 2024 |
| Contact name |
Jinyang Lyu |
| E-mail(s) |
lvjy890727@sdu.edu.cn
|
| Organization name |
Shandong University
|
| Department |
School of Life Sciences
|
| Street address |
BinHai road
|
| City |
Qingdao |
| State/province |
Shandong |
| ZIP/Postal code |
266237 |
| Country |
China |
| |
|
| Platform ID |
GPL26077 |
| Series (1) |
| GSE253343 |
The TaSnRK1-TabHLH489 module integrates brassinosteroid and sugar signaling to regulate grain length in bread wheat |
|
| Relations |
| BioSample |
SAMN39459278 |
| SRA |
SRX23231220 |
