|
| Status |
Public on Nov 30, 2011 |
| Title |
High fat diet mouse2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
adipose tissue from high fat diet mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Adipose tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using guanidinium thiocyanate following manufacturer's instructions
|
| Label |
Hy3
|
| Label protocol |
Labelling was performed using the miRCURY LNA™ microRNA Power Labeling Kit, according to the manufacturer protocol http://www.exiqon.com/ls/Documents/Scientific/miRCURY-LNA%E2%84%A2-microRNA-Array-v11-other-species-manual.pdf)
|
| |
|
| Channel 2 |
| Source name |
adipose tissue from standard fat diet mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Adipose tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using guanidinium thiocyanate following manufacturer's instructions
|
| Label |
Hy5
|
| Label protocol |
Labelling was performed using the miRCURY LNA™ microRNA Power Labeling Kit, according to the manufacturer protocol http://www.exiqon.com/ls/Documents/Scientific/miRCURY-LNA%E2%84%A2-microRNA-Array-v11-other-species-manual.pdf)
|
| |
|
| |
| Hybridization protocol |
Hybridization was performed according to the manufacturer protocol (http://www.exiqon.com/ls/Documents/Scientific/miRCURY-LNA%E2%84%A2-microRNA-Array-v11-other-species-manual.pdf)
|
| Scan protocol |
The miRCURY™ LNA array microarray slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA)
|
| Description |
Technical replicate 2 of 2.
|
| Data processing |
Image analysis was carried out using the ImaGene 8.0 software (BioDiscovery, Inc., USA). LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal.
|
| |
|
| Submission date |
Sep 27, 2011 |
| Last update date |
Nov 30, 2011 |
| Contact name |
Apostolos Zaravinos |
| E-mail(s) |
zaravinos.apostolos@ucy.ac.cy
|
| Organization name |
University of Cyprus
|
| Department |
Department of Biological Sciences
|
| Lab |
Molecular Medicine Research Center (MMRC)
|
| Street address |
Kallipoleos 75
|
| City |
Nicosia |
| State/province |
Nicosia |
| ZIP/Postal code |
1678 |
| Country |
Cyprus |
| |
|
| Platform ID |
GPL7723 |
| Series (1) |
| GSE32419 |
MicroRNA expression differences in adipose tissue between high and low fat diet mice |
|
