|
| Status |
Public on Jul 01, 2012 |
| Title |
ovary np |
| Sample type |
RNA |
| |
|
| Source name |
ovary tissue of none pregnant sow
|
| Organism |
Sus scrofa |
| Characteristics |
time point: none pregnant
tissue: ovary
|
| Treatment protocol |
The tissues were collected and washed in phosphate-buffered saline (PBS) immediately and then frozen in liquid nitrogen. All samples were stored at -80C until the RNA was extracted.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Affymetrix Fluidics Station 450 and Hybridization Oven 640 under standard conditions.
|
| Scan protocol |
Image processing was conducted using the Affymetrix GeneArray 3000 scanner.
|
| Description |
Gene expression data from none pregnant sow ovary
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. Knock out the smallest and biggst 2%, the average intensity for all the other probes on the array was scaled to 500.
|
| |
|
| Submission date |
Sep 28, 2011 |
| Last update date |
Jul 01, 2012 |
| Contact name |
ligang wang |
| E-mail(s) |
ligwang@126.com
|
| Organization name |
institute of animal sciense
|
| Street address |
2 Yuanmingyuan West Road
|
| City |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platform ID |
GPL3533 |
| Series (1) |
| GSE32438 |
Expression data from Porcine pregnant |
|
