|
Status |
Public on Oct 31, 2011 |
Title |
hypothyroid_ctrl_rep_3 |
Sample type |
RNA |
|
|
Source name |
hypothyroid_ctrl
|
Organism |
Mus musculus |
Characteristics |
strain: C57/Bl6 gender: male age: 9 months tissue: liver
|
Treatment protocol |
oral gavage
|
Growth protocol |
12h light-dark cycle, standard rodent chow and water ad libitum
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
Streptavidin-Cy3
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep-96 RNA amplificatio kit.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol on the BeadArray Reader
|
Description |
5613754020_C hypothyroid mouse control
|
Data processing |
Unmodified data obtained from GenomeStudio was background-subtracted and quantile-normalized using the lumi package within R. Only probes aligning to a single gene in the mouse genome as defined by Ensembl, had their signal values background corrected and quantile normalized using lumi.
|
|
|
Submission date |
Sep 28, 2011 |
Last update date |
Oct 31, 2011 |
Contact name |
Paul Webb |
E-mail(s) |
PWebb@tmhs.org
|
Phone |
713-441-2516
|
Organization name |
Methodist Hospital Reseach Institute
|
Department |
Diabetes Research Center
|
Lab |
Genomic Medicine
|
Street address |
6565 Fannin St. F8-060
|
City |
Houston |
State/province |
Texas |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platform ID |
GPL6887 |
Series (2) |
GSE32444 |
Identical gene regulation patterns of triiodothyronine (T3) and selective thyroid hormone receptor modulator GC-1 [Illumina] |
GSE32445 |
Identical gene regulation patterns of triiodothyronine (T3) and selective thyroid hormone receptor modulator GC-1 |
|