|
| Status |
Public on Oct 31, 2011 |
| Title |
hypothyroid_GC1_rep_3 |
| Sample type |
RNA |
| |
|
| Source name |
hypothyroid_GC1
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/Bl6
gender: male
age: 9 months
tissue: liver
|
| Treatment protocol |
oral gavage
|
| Growth protocol |
12h light-dark cycle, standard rodent chow and water ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Streptavidin-Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep-96 RNA amplificatio kit.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol on the BeadArray Reader
|
| Description |
5613754058_E
hypothyroid mouse GC1
|
| Data processing |
Unmodified data obtained from GenomeStudio was background-subtracted and quantile-normalized using the lumi package within R. Only probes aligning to a single gene in the mouse genome as defined by Ensembl, had their signal values background corrected and quantile normalized using lumi.
|
| |
|
| Submission date |
Sep 28, 2011 |
| Last update date |
Oct 31, 2011 |
| Contact name |
Paul Webb |
| E-mail(s) |
PWebb@tmhs.org
|
| Phone |
713-441-2516
|
| Organization name |
Methodist Hospital Reseach Institute
|
| Department |
Diabetes Research Center
|
| Lab |
Genomic Medicine
|
| Street address |
6565 Fannin St. F8-060
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE32444 |
Identical gene regulation patterns of triiodothyronine (T3) and selective thyroid hormone receptor modulator GC-1 [Illumina] |
| GSE32445 |
Identical gene regulation patterns of triiodothyronine (T3) and selective thyroid hormone receptor modulator GC-1 |
|
