|
| Status |
Public on Mar 01, 2012 |
| Title |
explants injected with ICD and MCI-HGR, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
explants from embryos injected with ICD and MCI-HGR
|
| Organism |
Xenopus laevis |
| Characteristics |
tissue origin: 2-cell stage embryos
tissue: epithelial explants
injected agent: synthetic mRNA encoding ICD along with MCI-HGR.
injected timepoint: 2-cell stage
explant timepoint: stage 9/10
harvest timepoint: equivalent of stage 14
|
| Treatment protocol |
Animal caps were cut, explanted and cultured in Danilchik's + 0.1% BSA from stage 9/10 until harvest at approximately stage 14.
|
| Growth protocol |
Xenopus embryos grown in 0.1X MMR until stage 9/10.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Proteinase K method.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to standard Affymetrix Protocols.
|
| |
|
| Hybridization protocol |
10ug of cRNA was hybridized to Xenopus laevis Affymetrix chips for 16hrs at 45C, then washed and stained using the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChip Scanner 3000 7G from Affymetrix
|
| Description |
Xenopus-ICD-MCI2
|
| Data processing |
Data was processed using Microarray Suite 5.0 software using Affymetrix default settings. Statistical analysis was later performed using Bullfrog software
|
| |
|
| Submission date |
Sep 28, 2011 |
| Last update date |
Mar 01, 2012 |
| Contact name |
Chris Kintner |
| E-mail(s) |
kintner@salk.edu
|
| Organization name |
Salk Institute
|
| Street address |
10010 N. Torrey Pines Rd.
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL10756 |
| Series (1) |
| GSE32452 |
Multicilin promotes centriole assembly and ciliogenesis during multiciliate cell differentiation. |
|
