|
| Status |
Public on Jan 30, 2024 |
| Title |
Rabbit ear hypertrophic scar, without treatment, rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Rabbit ear hypertrophic scar, without treatment
|
| Organism |
Oryctolagus cuniculus |
| Characteristics |
tissue: Rabbit ear hypertrophic scar
genotype: WT
treatment: without treatment
|
| Treatment protocol |
The three rabbits without treatment were taken as the control group (NS). The other rabbits with HS lesions were randomly divided into 3 groups (three rabbits in each group): HS, drug-loaded MNs, and 5-Fu injection. Administration started from the 22nd day after skin resection, once a week for 3 consecutive weeks (on the 22nd, 29th and 36th day). For NS and HS, no treatment was given. For drug-loaded MNs, one patch of drug-loaded MNs was used in each HS lesion. For 5-Fu injection, 0.1 mL of 5-Fu solution (120 μg) was injected in each HS lesion.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Rneasy mini plus kit (Qiagen) and used for the construction of sequencing libraries.
RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Sample 5
|
| Data processing |
CLC Genomics Workbench v 11.0.1
Sequence reads were trimmed for adaptor sequence/low-quality sequence using CLC genomic benchwork (parameter-Quality limit: 0.05)
Trimmed sequence reads were mapped to OryCun3.0 using CLC genomic benchmork (parameters-mismath cost: 2 insertion cost: 3 deletion cost: 3 length fracion: 0.8 similarity fraction: 0.8)
Read count extraction and normalization were performed using CLC genomic benchwork
Assembly: OryCun3.0
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample.
|
| |
|
| Submission date |
Jan 25, 2024 |
| Last update date |
Jan 30, 2024 |
| Contact name |
Huinan Suo |
| E-mail(s) |
suohuinan@outlook.com
|
| Phone |
+8615927609028
|
| Organization name |
Huazhong University of Science and Technology
|
| Department |
Dermatology
|
| Street address |
1277 Jiefang Avenue, Wuhan, Hubei Province, 430022, China
|
| City |
Wuhan |
| State/province |
Hubei Province |
| ZIP/Postal code |
430022 |
| Country |
China |
| |
|
| Platform ID |
GPL26786 |
| Series (1) |
| GSE254263 |
Effect of Endogenous Stimuli-Responsive Separating Microneedles to Inhibit Hypertrophic Scar on Rabbit Ear |
|
| Relations |
| BioSample |
SAMN39612116 |
| SRA |
SRX23449646 |
