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Sample GSM803775 Query DataSets for GSM803775
Status Public on Sep 29, 2011
Title OV:OVCAR_4 [118213hp133a11]
Sample type RNA
 
Source name Adenocarcinoma
Organism Homo sapiens
Characteristics tissue of origin: Ovarian
cell line: OVCAR_4
age: 42
Sex: F
prior treatment: CyPh/CsPt/Adr
epithelial: yes
ploidy: 3n+/-, Near-triploid 69+/- (58-80)
p53 mutation: WT
doubling time: 41.4
contributing institute: National Cancer Institute, NIH
contributing person: T.C. Hamilton
reference: Sem Ocol 11: 285-298, 1984
Treatment protocol No treatment
Growth protocol Cells grown in RPMI 1640 with 5% FBS (Bio Whittaker, not heat inactivated) and 1% L-glutamine. Cells not grown past 80% confluencey for attached cells, or 0,5 x 10-6 cells for suspended. Trypsinize (for attached cells) cells with 5 ml trypsin-EDTA per T162, for 15 min., at 370C. Spin down suspended cells, and resuspend in ~3ml growth media. Count cells. Pass 1x10-6 cells into new T162’s w 30 ml media. Repeat growth cycle until desired amount of cells are available. Cells are not grown past passage 20.
Extracted molecule total RNA
Extraction protocol Growth schedule prior to harvest: Grow cells to ~80 confluencey. Re-feed cells the day prior to harvest. Draw off media from attached cells, or spin down suspended cells. Rinse cells with ~10 ml 1x PBS. Purify using Quiagen Midi Kit (Cat # 75144) per instructions. In brief, lyse cells in 15 ml lysis buffer (w 10 ul fresh BME per ml) per 4 T162s. Scrape (attached) cells. Pipet into a 50 ml tube. Repeat with the next set of 4 flasks. When done, vortex 10 sec.. Draw lysate thru a 20 guage needle 12x’s. Freeze lysate at -80 deg C. Use a maximum of 100 x 10-6 cells per column.
Label biotin
Label protocol Done by Genelogic according to manufacturer's recommendations
 
Hybridization protocol Done by Genelogic according to manufacturer's recommendations
Scan protocol Affymetrix MicroArraySuite
Description Adenocarcinoma-moderate differentiation
Data processing Probes were normalized using RMA in Partek Genomics Suite version 6.2
 
Submission date Sep 29, 2011
Last update date Sep 29, 2011
Contact name Sudhir Varma
E-mail(s) sudhirv4rma@gmail.com
Organization name HiThru Analytics
Street address 1215 Wessex Pl
City Princeton
State/province NJ
ZIP/Postal code 08540
Country USA
 
Platform ID GPL570
Series (1)
GSE32474 Comparison between cell lines from 9 different cancer tissue (NCI-60) (Affymetrix U133 Plus 2.0)

Data table header descriptions
ID_REF
VALUE Probeset expression is in log2 units

Data table
ID_REF VALUE
1007_s_at 9.437788963
1053_at 7.951643944
117_at 4.901614189
121_at 9.21581459
1255_g_at 2.586452007
1294_at 5.692223072
1316_at 5.102294922
1320_at 5.090538025
1405_i_at 2.888266087
1431_at 3.232136011
1438_at 5.340279102
1487_at 8.588985443
1494_f_at 5.201663017
1552256_a_at 7.069705963
1552257_a_at 8.592071533
1552258_at 4.210630894
1552261_at 4.745614052
1552263_at 5.71592617
1552264_a_at 7.664468765
1552266_at 2.899405003

Total number of rows: 54675

Table truncated, full table size 1211 Kbytes.




Supplementary file Size Download File type/resource
GSM803775_118213hp133a11.cel.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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