|
| Status |
Public on Feb 02, 2024 |
| Title |
CM5 |
| Sample type |
RNA |
| |
|
| Source name |
genomic DNA from whole peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
vitamin c: 42,133
|
| Extracted molecule |
total RNA |
| Extraction protocol |
DNA was extracted from whole peripheral blood using the Promega (Madison, Wisconsin, EUA) automated system with magnetic beads following the manufacturer’s instructions
|
| Label |
Cy3 and Cy5
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
DNA from total leucocytes were firstly bisulfite converted using the EZ DNA Methylation Kit (Zymo Research Corporation, Irvine, CA, USA), then 500ng were hybridized in the Infinium Human Methylation 450k (IHM450K) BeadChip (Illumina, Inc., San Diego, CA, USA)
|
| Scan protocol |
Images were obtained using the Iscan system.
|
| Data processing |
Champ package for Rstudio was used to process data.
|
| |
|
| Submission date |
Jan 30, 2024 |
| Last update date |
Feb 02, 2024 |
| Contact name |
Isabella Harumi Yonehara Noma |
| E-mail(s) |
bellanoma@hotmail.com
|
| Organization name |
Universidade de São Paulo
|
| Street address |
Av Prof. Lineu Prestes, 580 - Bloco15
|
| City |
São Paulo |
| State/province |
Please select |
| ZIP/Postal code |
05508-900 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL34056 |
| Series (1) |
| GSE254646 |
RELATIVE ABUNDANCE OF phyla ACTINOBACTERIA AND VITAMIN C CONSUMPTION ARE ASSOCIATED WITH DNA METHYLATION. |
|
