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Status |
Public on Jul 04, 2024 |
Title |
skin construct, physio, 48 hours, DC1_2 |
Sample type |
SRA |
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Source name |
HFF-1, HaCaT
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Organism |
Homo sapiens |
Characteristics |
cell line: HFF-1, HaCaT cell type: immortalized human fibroblasts, immortalized human keratinocytes group: physio time point: 48 hours treatment: DC1
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Treatment protocol |
After 14 days, half of the constructs were perfused with TNF-α to mimic an inflammatory state. Electrostimulation is tested on normal and inflamed samples, to have unstimulated and stimulated samples, at 1 and 5V in direct current (DC), and in alternate current, at 10 and 100 Hz. Each condition was tested in three replicates, and sampling was done at three timepoints (baseline, 1 h and 48 h) following stimulation.
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Growth protocol |
Fibroblasts and keratinocytes were cocultured in a collagen matrix for 14 days, until the fibroblasts were embedded in the collagen matrix, and the keratinocytes over them
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Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using Quick-DNA/RNA™ Microprep Plus Kit from Zymo Research and store at -80°C RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
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Data processing |
CLC Genomics Workbench v 11.0.1 Sequence reads were trimmed for adaptor sequence/low-quality sequence using CLC genomic benchwork (parameter- Quality limit: 0.05) Trimmed sequence reads were mapped to GrCM38/mm10 using CLC genomic benchwork (parameters- mismath cost: 2 insertion cost: 3 deletion cost: 3 length fraction: 0.8 similarity fraction: 0.8) Read count extraction and normalization were performed using CLC genomic benchwork Assembly: mm10 Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
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Submission date |
Jan 31, 2024 |
Last update date |
Jul 04, 2024 |
Contact name |
Christine Nardini |
E-mail(s) |
christine.nardini.rsrc@gmail.com
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Organization name |
PICB
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Street address |
Via dei Taurini 9
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City |
Rome |
ZIP/Postal code |
00186 |
Country |
Italy |
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Platform ID |
GPL24676 |
Series (1) |
GSE254696 |
Differential Anti-Inflammatory Effects of Electrostimulation in a Standardized Setting |
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Relations |
BioSample |
SAMN39646202 |
SRA |
SRX23460536 |
Supplementary file |
Size |
Download |
File type/resource |
GSM8048962_13_S12.bamReadsPerGene.out.tab.gz |
372.0 Kb |
(ftp)(http) |
TAB |
SRA Run Selector |
Raw data are available in SRA |
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