|
| Status |
Public on Feb 27, 2024 |
| Title |
SHR+Allisartan3 |
| Sample type |
SRA |
| |
|
| Source name |
heart
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: heart
|
| Treatment protocol |
All animals were housed in a 12-hour light/dark room and provided with free access to tap water and chow feed in a laboratory environment. After acclimatization for 1 week in an on-site facility, the SHR+Allisartan group animals were orally administered Allisartanin 1 ml of distilled water for 34 weeks. The WKY and SHR groups were orally administered 1 ml of distilled water for 34 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer’s protocol.
The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Raw data (raw reads) were processed using Trimmomatic. The reads containing ploy-N and the low quality reads were removed to obtain the clean reads. Then the clean reads were mapped to reference genome using hisat2.
FPKM value of each gene was calculated using cufflinks, and the read counts of each gene were obtained by htseq-count. DEGs were identified using the DESeq(2012) R package functions estimateSizeFactors and nbinomTest. Pvalue < 0.05 and foldChange >2 or foldChange < 0.5 was set as the threshold for significantly differential expression.
Assembly: Rnor_6.0
Supplementary files format and content: Microsoft Excel file includes fpkm values for each Sample
|
| |
|
| Submission date |
Jan 31, 2024 |
| Last update date |
Feb 27, 2024 |
| Contact name |
Hao Wu |
| Organization name |
Tianjin Union Medical Center
|
| Department |
Department of Cardiology
|
| Street address |
No.190 Jiaoyuan Road, Hongqiao District
|
| City |
Tianjin |
| ZIP/Postal code |
300192 |
| Country |
China |
| |
|
| Platform ID |
GPL18694 |
| Series (1) |
| GSE254800 |
Transcriptome and Proteome Analysis Reveals Upregulation of GSTM2 by Allisartan Improving Cardiac Remodeling and Dysfunction in Hypertensive Rat |
|
| Relations |
| BioSample |
SAMN39711274 |
| SRA |
SRX23485573 |
