|
| Status |
Public on Feb 07, 2024 |
| Title |
Placenta Ethanol 4 Capture |
| Sample type |
SRA |
| |
|
| Source name |
Placenta
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Placenta
strain: C57/BL6
treatment: Ethanol
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using QIAamp DNA Micro Kit (Qiagen) following manufacturer's recommendations
Libraries were performed using SureSelectXT Methyl-Seq mouse Target Enrichment System (Agilent) following manufacturer's protocol
|
| |
|
| Library strategy |
Bisulfite-Seq |
| Library source |
genomic |
| Library selection |
RANDOM |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Sequence reads were trimmed using Trimmomatic (v0.36)
Alignment and methylation counts were performed with Bismark (v0.18.1)
Assembly: mm10
Supplementary files format and content: tab-delimited files including CpG sites coordinate, coverage, methylation values
Supplementary files format and content: Input files for the methylKit package
|
| |
|
| Submission date |
Feb 01, 2024 |
| Last update date |
Feb 07, 2024 |
| Contact name |
Serge McGraw |
| E-mail(s) |
Serge.mcgraw@umontreal.ca
|
| Organization name |
CHU Ste-Justine Research Center/Université de Montréal
|
| Lab |
McGraw Lab
|
| Street address |
3175 Côte Sainte-Catherine, office 5.17.005
|
| City |
Montreal |
| State/province |
Qc |
| ZIP/Postal code |
H3T 1C5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE254845 |
Sex-Specific DNA Methylation and Gene Expression Changes in Mouse Placentas After Early Preimplantation Alcohol Exposure[Methyl-Seq] |
| GSE254847 |
Sex-Specific DNA Methylation and Gene Expression Changes in Mouse Placentas After Early Preimplantation Alcohol Exposure |
|
| Relations |
| BioSample |
SAMN39732218 |
| SRA |
SRX23502121 |
