|
| Status |
Public on Mar 01, 2024 |
| Title |
Sequencing data of leaf of heat-resistant line under normal condition, replicate 2, bulkRNAseq |
| Sample type |
SRA |
| |
|
| Source name |
Leaf
|
| Organism |
Zea mays |
| Characteristics |
inbred line: Z58D
tissue: Leaf
treatment: 32℃ for 6h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using RNeasy Plant Mini kit (Vazyme, Nanjing, China). 2 ug of total RNA was used for the construction of sequencing libraries.
Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina and index codes were added to attribute sequences to each sample
Libraries for mRNA-seq were constructed and sequenced by Novogene Bioinformatics Technology Co., Ltd (Beijing, China)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Sequence reads were trimmed for adaptor sequence/low-quality sequence using cutadapt
trimmed reads mapped to reference genome by STAR.
Read count caculated by featurecounts
DEGs identified by DESeq2
Assembly: B73 V4
Supplementary files format and content: tab-delimited text files include raw count values for each Sample
|
| |
|
| Submission date |
Feb 01, 2024 |
| Last update date |
Mar 01, 2024 |
| Contact name |
chuangye Qi |
| E-mail(s) |
Chuangye.Qi@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Huffington Center
|
| Lab |
Zheng
|
| Street address |
6450 E Cullen St
|
| City |
HOUSTON |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL25410 |
| Series (1) |
| GSE254852 |
Heat-Resistant Inbred Lines Coordinate the Heat Response Genes Expression Remarkably in Maize |
|
| Relations |
| BioSample |
SAMN39734329 |
| SRA |
SRX23503179 |
