|
| Status |
Public on Sep 29, 2014 |
| Title |
Control NCTC8626 biological rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
P. aueruginosa grown for 4 hours in MHB
|
| Organism |
Pseudomonas aeruginosa |
| Characteristics |
strain: NCTC8626
agent: control
|
| Treatment protocol |
Test cells were treated with 10% (w/v) manuka honey for the 4 hour incubation period
|
| Growth protocol |
Cells harvested after 4 hours growth in MHB at 37°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of RNA was done following manufacturers instructions using SV Total RNA extraction Sytem, Promega
|
| Label |
biotin
|
| Label protocol |
As described by Affymetrix
|
| |
|
| Hybridization protocol |
As described by Affymetrix
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 7G Autoloader
|
| Description |
P. aueruginosa NCTC8626 C1
Gene expression data from untreated cells
|
| Data processing |
The data were analyzed with Affymetrix Expression Console using the RMA algorithm
|
| |
|
| Submission date |
Oct 03, 2011 |
| Last update date |
Sep 29, 2014 |
| Contact name |
Peter Giles |
| E-mail(s) |
GilesPJ@Cardiff.ac.uk
|
| Organization name |
Cardiff University
|
| Street address |
Heath Park
|
| City |
CARDIFF |
| ZIP/Postal code |
CF14 4XN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL84 |
| Series (1) |
| GSE32572 |
Expression data from Pseudomonas aueruginosa NCTC8626 treated with and without manuka honey |
|
