|
| Status |
Public on Feb 26, 2024 |
| Title |
Liver, Mir483Pat-KO, rep3 (RNA-seq) |
| Sample type |
SRA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
Sex: female
developmental stage: E18.5
genotype: Mir483Pat-KO
index: SLX-22976.UDI0062rna
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted using a miRNeasy Mini Kit (Qiagen – 217004), according to manufacturer's protocol. Quantity and quality of RNA were assessed using an Agilent 2100 Bioanalyzer and RNA 6000 Nano Kits (Agilent – 5067-1511)
500 ng total RNA per sample was used to prepare libraries using Illumina's TruSeq RNA Library kit, according to manufacturer's protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Liver, Mir483Pat-KO, rep3 miR483_485
|
| Data processing |
STAR 2.5.0A was used to perform the alignment and gene level count with GRCm38/Ensembl 100
Assembly: GRCm38
Supplementary files format and content: Gene level count in tab-delimited format
|
| |
|
| Submission date |
Feb 21, 2024 |
| Last update date |
Feb 26, 2024 |
| Contact name |
Brian Yee Hong Lam |
| E-mail(s) |
yhbl2@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Metabolic Research Laboratories
|
| Street address |
Box 289 Addenbookes Hospital, Hills Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0QQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE256302 |
Expression profiling of mouse livers with deletion of paternally-expressed, imprinted Mir483 [RNA-seq] |
|
| Relations |
| BioSample |
SAMN40024830 |
| SRA |
SRX23689652 |
