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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 26, 2024 |
| Title |
Liver, iTgMir483, rep1 |
| Sample type |
SRA |
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| Source name |
liver
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| Organism |
Mus musculus |
| Characteristics |
tissue: liver
Sex: female
age: 15 weeks postnatal
genotype: over-expressor
library: SLX-22976.UDI0047rna
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a miRNeasy Mini Kit (Qiagen – 217004), according to manufacturer's protocol. Quantity and quality of RNA were assessed using an Agilent 2100 Bioanalyzer and RNA 6000 Nano Kits (Agilent – 5067-1511)
500 ng total RNA per sample was used to prepare libraries using Illumina's TruSeq RNA Library kit, according to manufacturer's protocol.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Description |
Liver, iTgMir483, rep1 CMVxmiR #6
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| Data processing |
STAR 2.5.0A was used to perform the alignment and gene level count with GRCm38/Ensembl 100
Assembly: GRCm38
Supplementary files format and content: raw gene level count in tab-delimited format
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| Submission date |
Feb 21, 2024 |
| Last update date |
Feb 26, 2024 |
| Contact name |
Brian Yee Hong Lam |
| E-mail(s) |
yhbl2@cam.ac.uk
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| Organization name |
University of Cambridge
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| Department |
Metabolic Research Laboratories
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| Street address |
Box 289 Addenbookes Hospital, Hills Road
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| City |
Cambridge |
| ZIP/Postal code |
CB2 0QQ |
| Country |
United Kingdom |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE256304 |
Expression profiling of mouse livers with inducible over-expression of microRNA Mir483 |
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| Relations |
| BioSample |
SAMN40025775 |
| SRA |
SRX23689673 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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