|
| Status |
Public on Feb 27, 2024 |
| Title |
Control ChIP-seq from whole organism (ENCLB177MOZ) |
| Sample type |
SRA |
| |
|
| Source name |
Caenorhabditis elegans strain CS152 (sma-3(wk30);qcIs[gfp::sma-3+rol-6]) hermaphrodite L2 larva 24 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using microinjection targeting C. ...
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
cell_type: whole organism
biomaterial_type: whole organisms
biosample encode accession: ENCBS723SCX (SAMN40085494)
collection date: not applicable
geographic location: not applicable
encode accession: ENCBS723SCX
note: Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to SMA-3
Sex: hermaphrodite
dev stage: L2 larva
age: unknown
donor_id: ENCDO022KND
lab: Valerie Reinke, Yale
source: Valerie Reinke
worm synchronization stage: L1 larva starved after bleaching
post synchronization time: 24
post synchronization time units: hour
link: ENCBS723SCX at ENCODE; https://www.encodeproject.org/ENCBS723SCX/
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
general protocol: https://www.encodeproject.org/documents/d89f19f3-31f8-4079-9635-5d3dd799471e/@@download/attachment/Reinke_Lab_ModENCODE_ChIP_protocol_%28revised%205-24-13%29_4_mg.pdf
other: https://www.encodeproject.org/documents/00810348-0004-45b6-bdf6-05b9f0b38ee3/@@download/attachment/258.pdf
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
https://www.encodeproject.org/experiments/ENCSR101VVI/
***************
biological replicate number: 1
technical replicate number: 1
description: This is the ChIP-seq control of transgenic worms expressing SMA-3-eGFP fusion proteins.
experiment encode accession: ENCSR101VVI
assay title: Control ChIP-seq
assembly: ce11, ce10
encode release date: 2016-05-10
lab: Valerie Reinke, Yale
library encode accession: ENCLB177MOZ
fragmentation methods: sonication (generic microtip)
|
| Data processing |
See GSM*_README.txt supplementary file linked below
|
| |
|
| Submission date |
Feb 24, 2024 |
| Last update date |
Feb 27, 2024 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL18245 |
| Series (1) |
| GSE256553 |
Control ChIP-seq from whole organism (ENCSR101VVI) |
|
| Relations |
| BioSample |
SAMN40085494 |
