|
| Status |
Public on Feb 27, 2024 |
| Title |
Control ChIP-seq from whole organism (ENCLB264HOL) |
| Sample type |
SRA |
| |
|
| Source name |
Caenorhabditis elegans strain OP656 (unc-119 (tm4063) III; wgIs656) mixed sex early embryo 45 minutes post synchronization using egg bleaching insertion targeting C. elegans hmbx-1
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
cell_type: whole organism
biomaterial_type: whole organisms
biosample encode accession: ENCBS335RPL (SAMN40084834)
collection date: not applicable
geographic location: not applicable
encode accession: ENCBS335RPL
note: Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to HMBX-1
Sex: pooled male and female
dev stage: early embryonic
age: unknown
donor_id: ENCDO501DLP
lab: Valerie Reinke, Yale
source: Valerie Reinke
worm synchronization stage: egg bleaching
post synchronization time: 45
post synchronization time units: minute
link: ENCBS335RPL at ENCODE; https://www.encodeproject.org/ENCBS335RPL/
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
general protocol: https://www.encodeproject.org/documents/d89f19f3-31f8-4079-9635-5d3dd799471e/@@download/attachment/Reinke_Lab_ModENCODE_ChIP_protocol_%28revised%205-24-13%29_4_mg.pdf
other: https://www.encodeproject.org/documents/1c71c0f0-f8bb-44dd-bfd5-73b62a552516/@@download/attachment/2016-1598.pdf
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
https://www.encodeproject.org/experiments/ENCSR318RVX/
***************
biological replicate number: 1
technical replicate number: 1
description: This is the ChIP-seq control of transgenic worms expressing HMBX-1-eGFP fusion proteins.
experiment encode accession: ENCSR318RVX
assay title: Control ChIP-seq
assembly: ce11, ce10
encode release date: 2016-10-27
lab: Valerie Reinke, Yale
library encode accession: ENCLB264HOL
fragmentation methods: sonication (generic microtip)
|
| Data processing |
See GSM*_README.txt supplementary file linked below
|
| |
|
| Submission date |
Feb 24, 2024 |
| Last update date |
Feb 27, 2024 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL22765 |
| Series (1) |
| GSE256956 |
Control ChIP-seq from whole organism (ENCSR318RVX) |
|
| Relations |
| BioSample |
SAMN40084834 |
